DNA

Part:BBa_K1732002

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-07)

CDcel

CDcel domain allows for the gene of interest to synthesize proteins that will be secreted into the media. It is a cellulase catalytic domain that consists of a N-terminus that can be utilized as a carrier for extracellular production of recombinant proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 912
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 652


References D. Gao, S. Wang, H. Li, H. Yu, Q. Qi. "Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli". Microbial Cell Factories.

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