Composite

Part:BBa_K173022:Design

Designed by: Lorenzo Pasotti, Giulio Chiesa, Manuel Lupotto, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-16)

adhB and pdc inducible system (HSL)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2754
    Illegal AgeI site found at 3960
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


Design Notes

We used BioBrick Standard Assembly.


Source

BBa_F2620, iGEM Headquarters;

BBa_K173020, UNIPV-Pavia iGEM 2009 team.

References

[1] Barry Canton, Anna Labno, and Drew Endy. Refinement and standardization of synthetic biological parts and devices. Nature biotechnology, 26(7):787-793, 2008.

[2] Zhanchao Wang, Ming Chen, Yuquan Xu, Shuying Li, Wei Lu, Shuzhen Ping, Wei Zhang and Min Lin, "An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose". Biotechnology Letters, Volume 30, Number 4 / April, 2008.

[3] L O Ingram, T Conway, D P Clark, G W Sewell, and J F Preston, "Genetic engineering of ethanol production in Escherichia coli". Appl Environ Microbiol. 1987 October; 53(10): 2420–2425.

[4] Y A Osman and L 0 Ingram, "Zymomonas mobilis mutants with an increased rate of alcohol production". Applied and Environmental Microbiology, July 1987, p. 1425-1432 53, No. 7.

[5] L P Yomano, S W York, S Zhou, K T Shanmugam and L O Ingram, "Re-engineering Escherichia coli for ethanol production". Biotechnology Letters, Volume 30, Number 12 / December, 2008.

[6] Rainer Kalscheuer, Torsten Stolting and Alexander Steinbuchel, "Microdiesel: Escherichia coli engineered for fuel production". Microbiology 152 (2006), 2529-2536.