Coding
adhB

Part:BBa_K173017

Designed by: Lorenzo Pasotti, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

alcohol dehydrogenase II from Z. mobilis (codon optimized for E. coli)

Alcohol dehydrogenase II (adhB) catalyses the acetaldehyde reduction to ethanol. This enzyme, as reported in literature, is also able to work in the opposite direction, oxidizing ethanol to acetaldehyde.

This enzyme comes from Zymomonas mobilis ethanologenic bacterium and performs an essential step of alcoholic fermentation (see figure). adhB can be used to engineer a heterologous fermentation pathway together with pdc gene (BBa_K173016).

The coding sequence of this gene has been optimized for Escherichia coli codon usage.

Fermentation pathway of Z. mobilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None