Coding
pdc

Part:BBa_K173016

Designed by: Lorenzo Pasotti, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

pyruvate decarboxylase from Z. mobilis (codon optimized for E. coli)

Pyruvate decarboxylase (pdc) catalyses the decarboxylation of pyruvic acid to acetaldehyde and carbon dioxide. To do this, two thiamine pyrophosphate (TPP) and two magnesium ions are required as a cofactors. It is also called 2-oxo-acid carboxylase, alpha-ketoacid carboxylase, and pyruvic decarboxylase.

This enzyme comes from Zymomonas mobilis ethanologenic bacterium and performs an essential step of alcoholic fermentation (see figure). pdc can be used to engineer a heterologous fermentation pathway together with adhB gene (BBa_K173017).

The coding sequence of this gene has been optimized for Escherichia coli codon usage.

Fermentation pathway of Z. mobilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 480
    Illegal AgeI site found at 1686
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None