Composite

Part:BBa_K173006

Designed by: Lorenzo Pasotti, Letizia Diamante, Susanna Schiavi, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

tetR inverter - beta-galactosidase generator

This composite part can be used as a measurement system for beta-galactosidase expression: tetR promoter (BBa_R0040) acts as a constitutive promoter because tetR (BBa_C0040) is not expressed and so lacZ (BBa_I732005) is always transcribed.

If a promoter is assembled upstream, tetR expression can be modulated by the strength of the promoter and this device can be induced by [http://openwetware.org/wiki/ATc aTc] which binds tetR and inactivates it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None