Translational_Unit
yeast uS12

Part:BBa_K1729001:Design

Designed by: Dani Bauhan and Richard Anthony   Group: iGEM15_RHIT   (2015-09-14)


Yeast mitochondrial ribosomal protein MRPS12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this part is designed for expression in yeast, and the Kozak sequence includes the first nucleotides of the coding sequence, it was cloned as a translational unit.


Source

The sequence is from the S. cerevisiae genome.

References