Composite

Part:BBa_K1727006:Design

Designed by: Ying Kuan   Group: iGEM15_TCU_Taiwan   (2015-09-05)


Lac operator+RBS+Signal peptide+Signiferin+Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 174
    Illegal PstI site found at 168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BamHI site found at 162
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 174
    Illegal PstI site found at 168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 174
    Illegal PstI site found at 168
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PQE


Source

Signiferin with signal peptide is artificial synthesis.

References