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Composite

Part:BBa_K1727004:Design

Designed by: Ying Kuan   Group: iGEM15_TCU_Taiwan   (2015-09-05)


LAC operator+RBS+Signal peptide with Epinicidin-1+Terminater


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 27
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 27
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 27
    Illegal BglII site found at 270
    Illegal BamHI site found at 60
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 27
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 27
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 213


Design Notes

We use PQE60


Source

Epinecidin-1 is comes from Epinephelus coioides

References