Regulatory
PlsiR

Part:BBa_K1725400:Design

Designed by: Andrey Filipov   Group: iGEM15_Glasgow   (2015-09-08)

PlsiR (Promoter of light and stress integrating response Regulator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 456


Design Notes

PlsiR was ordered as a G-block gene fragment. The sequence submitted to the registry differs from the sequence found in the Synechocystis genomic DNA. Thymine at position 58 was changed to adenine to eliminate a SpeI restriction site.



Source

Synechocystis sp. PCC6803

References