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Composite

Part:BBa_K1725220:Design

Designed by: Charlotte Flynn   Group: iGEM15_Glasgow   (2015-08-10)


LuxABG with pBAD and LuxCDE with R00N1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6245
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1608
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3156
    Illegal BsaI.rc site found at 5630
    Illegal SapI site found at 961


Design Notes

None


Source

Vibrio fischeri regulatory protein LuxR (luxR) gene

References