![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
P-phlF
Part:BBa_K1725000:Design
Designed by: Mhairi Davidson Group: iGEM15_Glasgow (2015-08-06)
PhlF repressible promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
Stanton et al., (2014) overlapped the operator sequence for the PhlF repressor over the -10 and -35 sites of BBa_J23119, a strong, constitutive Anderson family promoter, meaning when the repressor binds to its operator sequence sigma factor cannot recognise the promoter (and therefore attract RNA polymerase) and transcription cannot start.
Source
From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html