Part:BBa_K1722007:Design
hUPll+AckRS Composite
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1622
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 811
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The AckRS gene that is achieved from Shenzhen Second People's Hospital has two EcoR1 restriction enzyme cutting sites in its sequence. We mutated them and designed primers to amplified both hUPll and AckRS from psi-Check2 vector. Then 3A Assembly was used to construct these two gene in pSB1C3.
Source
We achieved both hUPll promoter and AckRS from Shenzhen Second People's Hospital.
References
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[3]Moll R, Wu XR, Lin JH, Sun TT. Uroplakins specific membrane proteins of urothelial umbrella cells as histological markers of metastatic transitional cell carcinomas. Am J Pathol. 1995;147:1383–1397.
[4]Zhu H J, Zhang ZQ, Zeng XF, et al. Cloning and analysis of human uroplakin II promoter and its ap plication for gene therapy in bladder cancer[J] Cancer Gene Ther, 2004, 11: 263-272
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[7] Veronika F, Milan V, Sabine S. Structural basis for the site-specific Incorporation of Lysine Derivatives into Proteins. Plos One, 2014, 9(4): 1-7