Composite

Part:BBa_K1714007:Design

Designed by: Hiroki ONO   Group: iGEM15_HokkaidoU_Japan   (2015-09-17)

pBad/araC-RBS B0034-Ag43 thanatin 4mer as alpha domain-double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1684
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1966
    Illegal NgoMIV site found at 2476
    Illegal NgoMIV site found at 2497
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1413
    Illegal AgeI site found at 1485
    Illegal AgeI site found at 1557
    Illegal AgeI site found at 1629
    Illegal AgeI site found at 2236
    Illegal AgeI site found at 2650
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This part has 6 PstI sites so you shoud not digest this part by PstI.


Source

This part is derived from BBa_K759001 and it contains thanatin-Asp fragment that is synthesized by IDT.


References

Seiichi Taguchi, Kanako Kuwasako, Atsushi Suenaga, Miyuki Okada and Haruo Momose, Functional Mapping against Escherichia coli for the Broad-Spectrum Antimicrobial peptide, Thanatin, Based on an In Vivo Monitoring Assay System, J. Biochem. 2000, 128:745-754 van der Woude, Marjan W Henderson, Ian R. Regulation and function of Ag43 (flu). Annu. Rev. Microbiol. 2008, 62:153–69 Glen C. Ulett, Jaione Valle, Christophe Beloin, Orla Sherlock, Jean-Marc Ghigo and Mark A. Schembri. Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract Infect. Immun. 2007, 75(7):3233. DOI: 10.1128/IAI.01952-06