Composite

Part:BBa_K1713013

Designed by: Yibo Wu   Group: iGEM15_HZAU-China   (2015-09-17)

Plac/ara-1+B0032+lacI(+LVA)

The hybrid promoter is activated by Arabinose in the presence of Arac protein and suppressed by lacI protein in tha absence of IPTG.

Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1276
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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