Generator

Part:BBa_K1713005:Design

Designed by: Xudong Luo   Group: iGEM15_HZAU-China   (2015-09-14)

Plac/ara-1+strong RBS+araC(+LVA)+double terminator+Plac/ara-1+strong RBS+GFP(+LVA)+double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
    Illegal BamHI site found at 1241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1985


Design Notes

N/A

Source

The promoter was standardized based on a earliest paper of 1997 while other parts were all from iGEM distribution.

References

Stricker J, Cookson S, Bennett M R, et al. A fast, robust and tunable synthetic gene oscillator[J]. Nature, 2008, 456(7221): 516-519.