Generator

Part:BBa_K1713001

Designed by: Xudong Luo   Group: iGEM15_HZAU-China   (2015-09-14)

Plac/ara-1+strong RBS+LacI(+LVA)+double terminator

The hybrid promoter ( Plac/ara-1 ) can be activated by the AraC protein in the presence of arabinose and so that Lacl produced. And then the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).

Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1275
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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