Regulatory

Part:BBa_K1709006:Design

Designed by: Astrid Deryckere   Group: iGEM15_KU_Leuven   (2015-09-18)


RBS-LuxI-His-RBS-LuxR-E-Term-Plux


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1502


Design Notes

For the ease of cloning, the sequence was devided in multiple gBlocks. Therefor, there is a mistake in the composite part sequence since the scar is only partly present.


Source

The Lux operon originates from V. Fischeri.

References