Coding

Part:BBa_K1709004:Design

Designed by: Astrid Deryckere   Group: iGEM15_KU_Leuven   (2015-09-18)


LuxR-E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 774
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 9 bp were exchanged to create unique restriction sites and to optimize DNA folding.


Source

Sequence is originating from V. fischeri. Here, the sequence from BBa_I0462 was used and optimized.

References