Device

Part:BBa_K1698003:Design

Designed by: Brandon Malone   Group: iGEM15_Cork_Ireland   (2015-09-18)


BaseHunter : Activated to become detector for 32bp target sequence on SRY Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706
    Illegal SapI site found at 920


Design Notes

We had to make sure a target was used which didn't contain any illegal sites for biobricks. Also had to make sure target would not form hairpin structures or have a high GC content


Source

Part was ordered from IDT and cloned into a BBa_K584001 plasmid backbone

References