Coding

Part:BBa_K1689014:Design

Designed by: Wei weijia   Group: iGEM15_Peking   (2015-09-16)

dCas9-Clact


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Delta alpha segment of LacZ is fused with dCas9, with a 6xhis tag for protein purification.


Source

E.coli

References