Coding

Part:BBa_K1680021

Designed by: Nicolai von Kuegelgen   Group: iGEM15_Tuebingen   (2015-09-17)

Dronpa caged Cre with NLS


This part contains the protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa part:BBa_K1680006 using a 6 amino acid Ser/Gly-linker part:BBa_K1680000 and the scar (Ser/Thr) as spacer. The construct has an N-terminal NLS sequence part:BBa_K1680004. In the expressed construct the two Dronpa domains should form multimers leading to an inactivated Cre recombinase that is reversibly activatable by Dronpa illumination (see Zhou et al 2012).

This Part is cloned and shipped in the pTUM104 (Part:BBa_K801004) and thereby galactose inducible in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 298
    Illegal BglII site found at 2032
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1154
    Illegal SapI site found at 704
    Illegal SapI site found at 1751


[edit]
Categories
//cds
//cds/reporter/chromoprotein
//cds/reporter/gfp
//chassis/eukaryote/yeast
//function/recombination/cre
//function/reporter/fluorescence
Parameters
colorGreen