Coding

Part:BBa_K1680017:Design

Designed by: Nicolai von Kügelgen   Group: iGEM15_Tuebingen   (2015-09-17)

Cre-Dronpa Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 449
    Illegal SapI site found at 1046


Design Notes

Assembled using 3A assembly (RFC25).

Source

gBlock designed by Team Tuebingen.

References

Zhou, Xin X., et al. "Optical control of protein activity by fluorescent protein domains." Science 338.6108 (2012): 810-814.