Coding

Part:BBa_K1680013

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-17)

NDegron

This part contains the protein coding region for an NDegron as described by Taxis et al. 2009. The NDegron enhances protein turnover by destabilising the protein it is fused to upon cleavage by TEV protease. The part is supplied in RFC25. This part was intended as a backup for our project, due to time restraints we did not use it.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/eukaryote/yeast
//proteindomain/cleavage
//proteindomain/localization
Parameters
None