Part:BBa_K1678006:Experience

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Applications of BBa_K1678006

The part BBa_K1678006 uses BBa_K1678004 constitutive strong promoter to express mCerulean codon optimized for L. plantarum. The promoter in optimized for L. plantarum, but also works in E.coli.

We characterized the functioning of this promoter in E.coli. This promoter was put in front of mCerulean fluorescent protein, ligated into pSB1C3, and transformed into E.coli. The transformants were found to be fluorescing blue and were re-streaked with a non-fluorescent control on an LB agar plate.

Liquid cultures were made for both fluorescent and control cells, and were visualized in a transilluminator.

fluorescense from 3 colonies in liquid cultures was measured in triplicates in a Tecan Infinite 200 plate reader, and compared with controls. The reading were adjusted for absorbance and plotted with a 95% confidence interval error bars (N=9).

The part was sequenced, and no mutations were found.

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