Regulatory

Part:BBa_K1678005:Design

Designed by: Antoine Vigouroux   Group: iGEM15_Paris_Bettencourt   (2015-09-16)


Promoter from BBa_J23119 followed by 4 orthogonal LoxP sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Spacer have been added between the LoxP sites to allow for primer binding.


Source

DNA synthesis

References