Coding

Part:BBa_K1677103:Design

Designed by: Mackenzie Labine-Romain   Group: iGEM15_BABS_UNSW_Australia   (2015-08-20)


Invasin with LysM signal peptide (Lactococcus)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1516
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was assembled by overlap PCR of 3 IDT gBlocks, and certain codons were changed to allow for this format of DNA synthesis.



Source

The invasin gene was originally source by the Warsaw 2009 team from the bacterium Yersinia something or other.


References