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Coding

Part:BBa_K1677100:Design

Designed by: Mackenzie Labine-Romain   Group: iGEM15_BABS_UNSW_Australia   (2015-08-06)


Listeriolysin (lactococcus)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1621
    Illegal PstI site found at 1635
    Illegal NotI site found at 7
    Illegal NotI site found at 1628
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1621
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1621
    Illegal PstI site found at 1635
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was assembled by overlap PCR of two IDT gBlocks, and certain codons were changed to allow for this format of DNA synthesis.


Source

The LLO gene was originally source by the Warsaw 2010 team from the bacterium Listeria monocytogenes.

References