Coding
Part:BBa_K1676097:Design
Designed by: Ooi Kean Hean Group: iGEM15_NTU-Singapore (2015-09-18)
Mutant 1 of Lactate Permease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 938
Design Notes
Primers were designed to have the start and stop codon so that they will not be mutated during PCR
Source
Random mutagenesis was carried out via PCR to obtain the mutants.