Composite
Part:BBa_K1676081:Design
Designed by: Ooi Kean Hean Group: iGEM15_NTU-Singapore (2015-09-18)
Mutant 5 of LDH and wildtype LDP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 797
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2191
Design Notes
Primers were designed to have the start and stop codon so that they will not be mutated during PCR
Source
Wild type LDH and LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDH. PCR fragments carrying the mutants were then ligated to the wild type LDP to make a complete operon.