Reporter

Part:BBa_K1676046

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-16)

GFP Reporter with Mutant RBS 4GA

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the forth base pair. The base pair G is substituted to A. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.

The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.

This reporter can also be derived from the existing construct, BBa_J69511.

The characterization is shown below,

Growth curve:

456.png

GFP Reading:

Gfp456.png

It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


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Categories
Parameters
None