Coding
3alpha-HSD

Part:BBa_K1674001:Design

Designed by: Liron Abrahami   Group: iGEM15_Technion_Israel   (2015-09-13)


3alpha-hydroxysteroid dehydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Making this part compatible with the iGEM standards required changing base pairs in three restriction sites: EcoRI and two PstI.

Source

AKR1C9, originated from rat liver, is the isoform that has been Chosen for this part [1]. The nucleotide sequence for this isoform was taken from an article [2]

References

  1. Penning TM, Jina Y, Heredia VV, Lewis M, Structure–function relationships in 3-hydroxysteroid dehydrogenases: a comparison of the rat and human isoforms, Journal of Steroid Biochemistry & Molecular Biology 85 (2003) 247–255.
  2. Cheng KC, White PC, Qin KN, Molecular cloning and expression of rat liver 3 alpha-hydroxysteroid dehydrogenase, Mol Endocrinol. 1991 Jun;5(6):823-8.