Protein_Domain
SP-aprE

Part:BBa_K1674000:Design

Designed by: Liron Abrahami   Group: iGEM15_Technion_Israel   (2015-09-04)

Secretion signal peptide originated from the B.subtilis extracellular protease AprE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The base pairs sequence determined by us, using the optimized codons for B.subtilis.

Source

The secretion signal peptide originated from the B.subtilis extracellular protease AprE. The amino acid sequence of the secretion signal peptide has been taken from an article [1].

References

  1. Brockmeier U (2006) New strategies to optimize the secretion capacity for heterologous proteins in Bacillus subtilis.PhD thesis, Biowissenschaften der Ruhr-Universita ̈t, Bochum
  2. Kolkman, Marc A. B., René van der Ploeg, Michael Bertels, et al. 2008 The Twin-Arginine Signal Peptide of Bacillus Subtilis YwbN Can Direct Either Tat- or Sec-Dependent Secretion of Different Cargo Proteins: Secretion of Active Subtilisin via the B. Subtilis Tat Pathway. Applied and Environmental Microbiology 74(24): 7507–7513.
  3. Wang, Guangqiang, Haiqin Chen, Hao Zhang, Yuanda Song, and Wei Chen 2013 The Secretion of an Intrinsically Disordered Protein with Different Secretion Signals in Bacillus Subtilis. Current Microbiology 66(6): 566–572.
  4. Bacillus subtilis subsp. subtilis str. 168 [gbbct]: 1 CDS's (256 codons) "http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=224308"
  5. Moszer, I., Rocha, E. P., & Danchin, A. (1999). Codon usage and lateral gene transfer in Bacillus subtilis. Current opinion in microbiology, 2(5), 524-528.
  6. JAVA CODON ADAPTATION TOOL "http://www.jcat.de"