Reporter

Part:BBa_K1670003:Design

Designed by: Christoph Schilling   Group: iGEM15_Manchester-Graz   (2015-08-25)

aidA-Promoter + mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 752
    Illegal AgeI site found at 864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 189


Design Notes

strong ribosome binding site mRFP based on BBa_K1362461(2 silent mutations at 213 and 435 T->A to delete HindIII recognition sites) CepR-binding box in promoter


Source

Synthetic gene fragment, according to genome sequencing from Burkholderia cenocepacia GenBank: HG938372.1