Coding

Part:BBa_K1669004:Design

Designed by: Nina Jerala   Group: iGEM15_Slovenia_HS   (2015-09-13)


BdhB + His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1021


Design Notes

C-terminal His-tag.


Source

Sequence retrieved from GenBank and codon optimized for expression in E. coli

References