Composite
Part:BBa_K1660009:Design
Designed by: Dai Yuanyi Group: iGEM15_BNU-CHINA (2015-09-08)
J23100-B0034-rMpL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 56
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 113
Illegal AgeI site found at 209
Illegal AgeI site found at 424 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We optimized the codon sequence for E. coli. and add a constitutive promoter J23100 upstream the coding sequence of rMpL, so that this parts can express rMpL lectin continuously.
Source
It comes from Macrolepiota procera cDNA.