Composite

Part:BBa_K1660008:Design

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-08)


J23100-B0034-bace16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 152
    Illegal NgoMIV site found at 515
    Illegal NgoMIV site found at 587
    Illegal NgoMIV site found at 839
    Illegal AgeI site found at 446
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add J23100, a constitutive promoter upstream the coding sequence of bace16, so that it can express Bace16.


Source

It comes from Bacillus nematocida.

References