Part:BBa_K1660004:Design

PcyA+Ho1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 816
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 369
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We used oe-pcr to assemble these parts together seamlessly.

Source

pcyA gene and ho1 gene both come from Synechocystis.