Device

Part:BBa_K165078:Experience

Designed by: Aaron Glieberman   Group: iGEM08_BrownTwo   (2008-10-29)

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Applications of BBa_K165078

In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional. After growing in 2% galactose/2%raffinose, under the confocal fluorescence microscope we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below:


Fluorotest.png

The mCherry on the activator is red in the nucleus, and the YFP is green in cytosol.

User Reviews

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