Regulatory
Part:BBa_K165031:Design
Designed by: Aaron Glieberman Group: iGEM08_BrownTwo (2008-10-28)
mCYC promoter plus LexA binding sites
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 149
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector
Source
Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.