Regulatory

Part:BBa_K165030:Design

Designed by: Aaron Glieberman   Group: iGEM08_BrownTwo   (2008-10-28)


mCYC promoter plus Zif268-HIV binding sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 41
    Illegal XhoI site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.


Source

Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.

References