Part:BBa_K165020

LexA Synthetic Activator (untagged)

This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the LexA DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Lex operator, activating transcription downstream.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Improvement by UCopenhagen2020

This biobrick was improved upon by the UCopenhagen 2020 team. The improvement consists of fusing the biobrick to a transmembrane domain linked by a flexible link containing a TEV protease cleavage site as well as adding an ER import signal. This makes the synthetic transcription factor localize to the membrane. If a TEV protease cleaves the biobrick, the synthetic transcription factor is able to relocate to the nucleus and induce expression of a reporter gene under control of a LexAop promoter. The improved biobrick can be found at BBa_K3617007.