Composite

Part:BBa_K1650048:Experience

Designed by: Oliver Schauer   Group: iGEM15_Marburg   (2015-09-18)


Experience of iGEM Marburg 2015

This part was used in the NUTRInity-PickUp-System.

Westernblot

Figure 1: Westernblot shows the presence of a His taged fusion protein consisting of Spycatcher-GFP
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Figure 2: Westernblot shows the binding of the GFP-spycatcher (BBa_K1650048) to CsgA-spytag (BBa_K1650047). Both constructs were cotransformed in the same cells. The band at 55kd is signifantly higher than the band of spycatcher-GFP-His alone at 35-40kd proving that spycatcher and spytag have bound intracellulary

Applications of BBa_K1650048

User Reviews

UNIQ2d250e78b55948a0-partinfo-00000001-QINU UNIQ2d250e78b55948a0-partinfo-00000002-QINU