Part:BBa_K1650048

Plac_RBS_spycatcher-GFP-His

Characterization

Westernblot

Figure 1: Westernblot shows the presence of a His taged fusion protein consisting of Spycatcher-GFP

7

Figure 2: Westernblot shows the binding of the GFP-spycatcher (BBa_K1650048) to CsgA-spytag (BBa_K1650047). Both constructs were cotransformed in the same cells. The band at 55kd is signifantly higher than the band of spycatcher-GFP-His alone at 35-40kd proving that spycatcher and spytag have bound intracellulary

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 448
  Illegal XhoI site found at 871
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1713