Composite
RBS oprF
Part:BBa_K1648047:Design
Designed by: Leung King Pong Group: iGEM15_Hong_Kong-CUHK (2015-05-31)
AvOprF with RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 493
Design Notes
Insert the oprF into B0034 using standard restriction sites.
Source
B0034 is from iGEM distribution kits. The oprF gene was obtained by PCR from the Azotobacter vinelandii strain DJ genome.