Part:BBa_K1648005:Experience

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Applications of BBa_K1648005

The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [K929102].

Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.

L: DNA ladder. Lane 2: GFP-nanobody.

This Part is added to Insertion Kit(K1648004), forming a new Part(K1648006), which is used to express GFP-nanobody on the membrane of magnetosome.

This Part were verified by double digestion (Fig. 2) and sequencing.

Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.

User Reviews

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