Part:BBa_K1648004:Experience

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Applications of BBa_K1648004

Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into A. vinelandii genome via random integration.

A GFP-nanobody(K1648005) is added to this part in order to express GFP-nanobody on the magnetosome. This new part is K1648006

This Part were verified by double digestion (Fig. 1).

Fig 1. The photo of 1% agarose gel electrophoresis showing PCR products of operons in Magnetosome Island (MAI). L: DNA ladder. Lane 1: mamHIEJKLMN. Lane 2: mamOPQRBSTU. Lane 3: mamPQRBSTU. Lane 4: mamYXZftsZ-like. Lane 5: mamGFDC. Lane 6: mamGFD. Lane 7: mms6.

RFP is inserted in Insertion Kit for biobrick standard. The Insertion kit with RFP is transformed in E. coli (DH5 alpha). Expression of RFP in Insertion is shown(Fig 2.).

Fig 2. Insertion kit with RFP(BBa_K1648004) is expressed in E. coli (DH5 alpha)

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