Composite

Part:BBa_K1648002:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-16)

Recombination Template for mamAB Operon with Promotor and Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 855
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 371
    Illegal AgeI site found at 890
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 616


Design Notes

We add a promotor and RBS before each template to initial the coding and a double terminator(B0015) behind each template to terminate the coding and ligate these parts together.

K1648002 history.png

Source

Magnetospirillum gryphiswaldense MSR-1 v2, complete genome

References