Composite

Part:BBa_K1642011:Design

Designed by: Can Yin   Group: iGEM15_SJTU-BioX-Shanghai   (2015-09-05)

Expression of Halorhodopsin under the control of Pdrak


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 783
    Illegal NgoMIV site found at 942
    Illegal NgoMIV site found at 1263
    Illegal AgeI site found at 1134
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Pdark(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters)

HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)

Pdark-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Desalination_Process)

Source

Overlap PCR from basic parts Up(BBa_K1642005), Pdark(BBa_K1026009), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).