Translational_Unit

Part:BBa_K1641042:Design

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-10)


Fusion protein of Cre-EGFP, with RBS at the beginning


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Cre-EGFP do not process ssra, and hence much stable in vivo. As a result, the enzymatic activity seems much higher than those with ssra. Yet, a tiny leakage express might happen even if linked to inductive promoter.


Source

Cre from BBa_K1179058 and EGFP from pBI121-EGFP is fused by overlap-PCR; RBS is added via PCR primer.

References