Generator

Part:BBa_K1641036:Design

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-12)


Inductive invertase generating vector - pInv-gen-Cre-EGFP-ssra


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1504
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

All invertase generators are constructed as following structure:

AraC+Pbad::RBS::invertase-EGFP(-ssra)::terminator


Source

This brick is constructed through cut-and-link of other bricks without risky sources, usually E. coli originated genes.

References